[Vascular endothelial injury induced by anti-endothelial cell antibody in allogeneic hematopoietic stem cell transplantation].

Objective To clarify the role of endothelial cells (ECs) injury induced by antiendothelial cell antibody (AECA) in allogeneic hematopoietic stem cell transplantation (allo-HSCT). Methods Serum immunoglobulin (IgG) from allo-HSCT recipients were purified and incubated with human umbilical vein vascular endothelium (HUVEC) in vitro, then the functional changes and cell apoptosis were tested. Results After incubation with AECA positive IgG, soluble adhesion molecules significantly elevated in culture supernatant. When concentration of IgG was 160, 320, and 640 μg/ml, concentrations of soluble intercellular adhesion molecule-1 in supernatant were statistically higher in AECA positive groups [(117.10±12.82) vs (78.17±4.90) pg/ml, (151.30±15.35) vs (89.46±6.02) pg/ml,(239.00±32.53) vs (127.80±13.86) pg/ml, P 0.05). Moreover, IgG from AECA positive samples did not change the proliferation or cell apoptosis. Conclusions AECA from allo-HSCT recipients dysregulates ECs' function in vitro, but do not induce apoptosis, which is valuable in the pathophysiology of graft-versus-host disease (GVHD) and other complications after allo-HSCT. Key words: Hematopoietic stem cell transplantation; Graft-versus-host disease; Anti-endothelial cell antibody; Human umbilical vein vascular endothelium; Apoptosis