Acute effects of tobacco smoke on human airway dendritic cells in vivo
2010
Airway dendritic cells (DCs) play a key role in smoke-related
lung diseases; however, the acute effects of tobacco smoke on human airway
DCs in vivo are unknown. A total of 16 smokers underwent bronchoalveolar lavage at two time-points:
directly after a 4-h period of nonsmoking (no smoke exposure); and
directly after a 4-h period during which eight cigarettes were smoked (acute
smoke exposure). Using flow cytometry, myeloid DCs (mDCs) and
plasmacytoid DCs (pDCs), as well as function-associated surface
molecules on mDCs, were analysed in bronchoalveolar lavage fluid (BALF)
and in blood. The numbers of macrophages, lymphocytes, neutrophils, eosinophils and pDCs
were unchanged in BALF following acute smoke exposure, as compared to no smoke
exposure. In contrast, there was a strong increase in mDC number in BALF and
a concomitant decrease in mDC number in blood following acute smoke exposure.
In addition, acute smoke exposure led to an increase in the expression of
the surface molecules blood dendritic cell antigen 1 and 4 and a decrease
in the expression of the lung homing receptor, CC chemokine receptor 5, on
mDCs in BALF. Acute tobacco smoke inhalation results in an immediate and selective recruitment
of mDCs into human airways, which might reflect the very early reaction of
the adaptive immune system to smoke exposure.
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