The influence of beta–thalassemia minor on glycosylated hemoglobin four measuring systems

Objective To investigate the Influence of beta–thalassemia minor on four different HbA1c detection systems. Methods All 65 blood samples from March 2014 to August 2014 were collected from Zhongshan Hospital of Sun Yat–sen University, and divided to normal control group(40 cases), no diabetic group(20 cases) and diabetic group(5 cases)combining with beta–thalassemia minor. The fresh mixed whole–blood samples were used for transferring value–assignment in order to improve the comparability of Bio–Rad variant ⅡTurbo, Primus Ultra2, Roche Modular PPI to Bio–Rad Variant Ⅱwhich was NGSP Ⅰlaboratory certificated. The whole–blood concentration of HbA1c were measured by four detection systems. Differences between normal control group and no diabetic group were compared using the Independent Samples T Test. Then Taking the Primus Ultra2 as comparable system and others as experimental system, the HbA1c results from no diabetic group and diabetic group were compared by the standardization NGSP Ⅰlaboratory and statistical techniques of consistency test. Results Compared with Variant Ⅱ detection system, after transferring value–assignment, deviations of Variant Ⅱ, Modular PPI and Variant Ⅱ Turbo were –6% to+ 6%. The HbA1c testing results from normal control group and no diabetic group had no statistical significance (P>0.05). Linear regression analysis demonstrated that the correlation coefficient of Primus Ultra2 with Variant Ⅱ, Modular PPI, Variant Ⅱ Turbo were 0.995, 0.999 and 0.995, respectively (P<0.01). The percentage deviation of the reference system and experimental system was –6.0% to+ 6.0%. Conclusion There was no obviously significant influence of beta–thalassemia minor on Bio–Rad Variant Ⅱ, Bio–Rad variant ⅡTurbo, Primus Ultra2, Roche Modular PPI detection systems.(Chin J Lab Med, 2015, 38: 691–695) Key words: beta–Thalassemia; Hemoglobin A, glycosylated