Antagonistic Bacillus cereus TC-1 Isolated from Solar Salt Work inSouthern India

Bacillus cereus TC-1 was isolated from condenser pond of manmade solar salt pan in Thamaraikulam, Tamilnadu, India effectively suppressed the shrimp bacterial pathogens of Vibrio harveyi, V. parahaemolyticus, V. anguillarum, V. alginolyticus and V. vulnificus by In vitro antagonistic assay at 9 to 15 mm of zone of inhibition. Phylogenetic analysis and evolutionary distance revealed that, B. cereus TC-1 was highly similar to that the Bacillus sp. and other B. cereus strains. Their optimum growth rate was ranged between of 4 to 6% of NaCl in the growth media. The alkaline protease production was significantly (P< 0.001) differed among the different NaCl enriched growth media. Based on the antagonistic activity of the B. cereus TC-1 against the shrimp pathogens and its antimicrobial factors, it may be used as probiotics and developing novel antimicrobial bioactive substances against aquatic pathogens.