Effect of water hardness/alkalinity and humic substances on the toxicity of peracetic acid to zebrafish embryos and pathogenic isolates

Abstract The prophylactic use of peracetic acid (PAA)-based disinfectants is becoming more popular in aquaculture due to rising concerns regarding sustainability, fish welfare and food safety. However, specific and effective PAA dosing protocols have not been developed to guide the aquaculture industry under diverse production conditions. In the present study, the effect of water hardness/alkalinity and humic substances (HS) on the toxicity of PAA to zebrafish Danio rerio embryos and the efficacy of PAA against the in vitro growth of Yersinia ruckeri and Saprolegnia parasitica was investigated. PAA concentrations that were safe to fish embryos demonstrated strong bactericidal, but limited fungistatic properties. In higher hardness/alkalinity water, or when HS was added, the same concentration of PAA resulted in a smaller pH decrease accompanied by a smaller increase of oxidation-reduction potential (ORP), and showed lower toxicity and weaker antimicrobial effects than in lower hardness/alkalinity waters. We suggest the determining factor of PAA toxicity and its antimicrobial capacity was likely ORP. At low hardness/alkalinity conditions, strong pH reduction (resulting in pH