Establishment of sleeve gastrectomy model in obese mice

Objective To establish a sleeve gastrectomy (SG) model in obese mice with high efficiency and high success rate. Methods Forty 6-week-old male C57BL/6J mice weighing (21.05±1.07) g were randomized into two groups: control group (mice were fed on regular chow diet, n=8) and high-fat diet group (mice were fed on high fat diet for 16 weeks to induce obesity, n=32). After 16 weeks, obese mice over 38 g were randomized into SG group (n=16) and sham operation group (n=8). The average body weight in SG group and sham group was (47.31±2.35) and (45.38±2.97) g respectively. The body weight, fasting blood glucose, and fasting insulin levels were measured at 8th week post-operation. The oral glucose tolerance test (OGTT) was used to evalue the insulin sensitivity. Results The body weight in the sham operation group began to rebound from the 6th day after surgery and was higher than the preoperative level at 8th week after surgery. The body weight in the SG group was decreased early after surgery, and was still significantly lower than the sham group after 8 weeks [(36.50±2.31) g vs. (46.20±2.05) g; P=0.001]. The fasting plasma glucose in the SG group was significantly lower than that in the sham group and the high-fat group (P=0.001, 0.001) after 8 weeks postoperatively, and fasting plasma glucose was (5.38±1.07), (7.84±0.80) and (8.62±1.31) mmol/L respectively. Fasting insulin in the SG group was significantly lower than that in the sham group and the high-fat group at 8th week after operation (P=0.001, 0.001), which was (205.75±42.80), (293.50±30.78), (327.88±46.23) mU/L. The results of OGTT and the insulin resistance index showed that SG could significantly improve insulin sensitivity in obese mice (P=0.015, 0.003). Conclusion This research will provide us a more reasonable and effective model for the study of the treatment mechanisms of SG. Key words: Obesity; Sleeve gastrectomy; Model, animal