Anti-inflammatory Effect of Coptidis Rhizoma

ABSTRACT Objectives： The present study investigated Inflammatory effect of Coptidis Rhizoma in lipopolysaccharide- exposed rats and Raw 264.7 cells. Methods： The plasma concentration of IL-1β, IL-6 and TNF-α peaked at 5 h after LPS injection, and the values of the Coptidis Rhizoma extract groups were lowe r than those of the control group. In the increment of cytokines concentration at 2 h and 5 h after LPS injection, the Coptidis Rhizoma groups were lower than that of control group. The plasm a concentration of IL-10 peaked at 5 h after LPS injection, and the values of the Coptidis Rhiz oma extract groups were higher than those of the control group. In the increment of cytokines conce ntration at 2 h and 5 h after LPS injection, the Coptidis Rhizoma groups were higher than that of control group. Liver cytokines measurement was done at 5 h after LPS injecti on. The concentration of liver IL-1β and IL-6 in the Coptidis Rhizoma groups was lower than that of the control group. The concentrations of liver TNF-α, and IL-10 showed no significant differences among all the treatment groups. Results： In the studies of lipopolysaccharide-exposed Raw 264.7 cells, t he concentration of IL-1β, IL-6 and TNF-α in the lipopolysaccharide-exposed cells groups was higher than that of control group (normal group), and in the lipopolysaccharide-exposed cells gro ups, these values showed a tendency to decrease in the Coptidis Rhizoma groups. The concentration o f IL-10 in the lipopolysaccharide- exposed cells groups was higher than that of control group (nor mal group), and in the lipopolysaccharide- exposed cells groups, the values showed a tendency to increase in the Coptidis Rhizoma groups. Conclusions： These results indicate that the Coptidis Rhizoma extracts have an functional material for Inflammatory activities.Key words : Anti-inflammatory effect, Coptidis Rhizoma, LPS shock, cytokines, Raw 264.7 cells