Gene sequencing method-based HLA-B27 genotyping method

The invention discloses a gene sequencing method-based HLA-B27 genotyping method, belonging to the technical field of biology. By comparing subtypes of B27-01, B27-02, B27-03, B27-04, B27-05, B27-07, B27-10 and B27-15 of an HLA-B27 gene and finding a keeping area thereof, a sequencing primer and a probe aiming at the subtypes of the HLA-B27 gene are designed, and HLA-B27 genotyping is realized by a method of combining fluorescent PCR with sequencing. The method is simple to operate, specific in typing, high in accuracy, monitorable in process, quick in result acquiring, higher in flux, free of an electrophoresis process and beneficial to clinic popularization.