Partial Characterization and Serological Relationship of a Tospo Virus Infecting Sunflower (Helianthus annuss. L) In South India.

A tospo virus infecting sunflower in India was characterized by host range studies, the virus was purified and polyclonal anti serum was produced. The virus infecting sunflower reacted with homologous antiserum, and PBNV antiserum is DAC-ELISA, and failed to react with TSWV, INSV, IYSV and PSNV. The purified virus resolved as four polypepticles species of 331, 58, 52, 31 kD in SDS PAGE. In electro blot immuno assay, all four polypeptides reacted with homologous antiserum, and only nucleocapsid protein of 31kD reacted with recombinant nucleocapsid antibodies raised against PBNV and none of the polypeptides reacted with TSWV, INSV and IYSV. The RNA from purified virus resolved into three bands of L RNA of 9 Kb, M RNA of 5 Kb and S RNA of 3.5 Kb in agarose gel electrophoresis.