Nano-liposomal entrapment of bioactive peptidic fraction from fish gelatin hydrolysate

Abstract This study guided the potential of nanoliposomal vesicles for the encapsulation of an active peptidic fraction from the fish skin gelatin by enzymatic hydrolysis using alcalase. The average particle size of peptide-loaded nanoliposomes was found to be in the range 134–621 nm, with a ζ-potential of 0.06–8.65 mV and a polydispersity index of 0.27–0.49, when the initial peptide content was 0–10 mg/ml. The FTIR analysis showed that there was an effective hydrophobic interaction as well as hydrogen bonding between phosphatidylcholine and peptidic fraction. The encapsulation efficiency (EE) was remarkably influenced by peptide concentration and the maximum EE (84.5%) was achieved with 1 mg/ml peptide concentration. A prolonged release of peptide from nanoliposomes was also observed as compared to free peptidic fraction. The results showed that the encapsulation of gelatin peptidic fraction using liposomal nanocarrier could represent a useful approach to overcome the issues associated with direct application of these antioxidant peptides in foodstuffs.