Efficient and safe transposable element integration system and application thereof

The invention belongs to the field of molecular biology, relates to an efficient and safe transposable element integration system and application thereof and further relates to a nucleic acid construction body and application thereof. The nucleic acid construction body concretely and sequentially comprises the following elements of a transposable element 5' terminal repetition sequence, a polyclone insertion site, a poly A tailing signal sequence, a transposable element 3' terminal repetition sequence, a transposase coding sequence and a promoter controlling the expression of transposase. The polyclone insertion site is used for inserting an exogenous gene coding sequence and a selective promoter for controlling the expression of exogenous genes in an operability mode. The poly A tailing signal sequence has a poly A tailing signal function in the forward and reverse directions, and the direction of an expression cassette of transposase is reverse to that of an exogenous gene expression cassette. The nucleic acid construction body can be used for efficiently and safely expressing mediated exogenous genes in host cells.