Development of ionic liquid assisted-synthesized nano‑silver combined with vascular endothelial growth factor as wound healing in the care of femoral fracture in the children after surgery

Abstract This investigation aimed to develop the silver (Ag) nanoparticles incorporated vascular endothelial growth factor (VEGF) for the improvement wound healing and reduce Aseptic necrosis in treatment of femoral fracture healing. The spherical shaped Ag nanoparticles with improved morphology have been effectively synthesized via microwave assisted method using ionic liquids 1-dodecyl-3-methylimidazolium chloride. The morphological structure and crystalline properties of Ag nanoparticles are analyzed by using UV, XRD and TEM-EDX analytical methods. The average grain size of the Ag nanoparticles is 20 nm, which was observed by defining the width of the (111) Bragg reflection with the Debye–Scherer formula and TEM results. The biological analyses confirmed that the Ag nanoparticles with VEGF molecules are promoted the cell adhesion and proliferation of Human mesenchymal stem cells (MSCs) cells. Ag NPs at appropriate concentrations have favorable biocompatibility to encourage cell activation properties like cell proliferation, cytokines release and chemotaxis. In the present study, our experimental results indicated that Ag NPs incorporated VEGF material are highly favorable to fracture healing and mainly as blood vessel repair. The surface morphology improved synthetic Ag NPs using ionic liquids has shown advantageous for cell activity and also improve the materials performances with VEGF for the regeneration of femoral fractures.