542PANALYSIS OF PROGRESSION FREE SURVIVAL IN THE NEW EPOC STUDY IN AN ALL RAS WILD-TYPE POPULATION

ABSTRACT Aim: The New EPOC study randomised KRAS exon 2 wild-type (WT) patients with resectable or suboptimally resectable colorectal liver metastases (CRLM) to receive chemotherapy with or without cetuximab before and after liver resection. KRAS status was determined using pyrosequencing in codons 12, 13 and 61. The trial demonstrated a detriment in progression free survival (PFS) from 20·5 to 14·1 months with the addition of cetuximab to chemotherapy (HR 1·48 95%CI 1·04-2·12 p = 0·03). Subsequently, benefit from epidermal growth factor inhibition in advanced disease was shown to be improved by further defining the population as all RAS WT (Douillard NEJM 2013). Methods: Samples of tumour from the primary colorectal and liver resections were obtained. Patients were further analysed using MiSeq for KRAS (codon 12, 13, 61, 117 and 146), BRAF (V600E), NRAS (12, 13, 61, 117 and 146), PIK3CA (547 and 1047) and EGFR S492R. Survival analyses were completed using the Kaplan-Meier method and the log-rank test. Results: To date 140 samples of primary tumour and 103 samples of CRLM have been analysed. Paired samples of primary tumour and CRLM were analysed for 61 patients. Further mutations were found in samples from 12 patients in the initial “KRAS WT” group, 8 KRAS (4 in codons previously analysed by pyrosequencing) and 4 NRAS. 3 mutations identified in CRLM were not in the matching primary. Analysis of the all RAS WT primary tumour population (chemo alone arm n = 53, chemo plus cetuximab arm n = 72) demonstrated a detriment in median PFS of 20 months to 15 months respectively (HR 1·4 95%CI 0·82-2·4 p = 0·22). 7 tumours were found to be BRAF mutated in all patients sequenced. Conclusions: In this study the addition of cetuximab to chemotherapy and surgery for operable CRLM in KRAS wild-type patients resulted in an inferior PFS. More stringent selection of an all RAS WT cohort did not alter the detriment observed. BRAF mutation is uncommon and likely reflects the selection of a relatively good prognosis cohort. Differences in mutational status between primary and metastasis were infrequent and could reflect either a treatment effect or clonal outgrowth. Initial pyrosequencing failed to detect Disclosure: J.A. Bridgewater: reports personal fees from Merck advisory board, Bayer advisory board, Sanofi-aventis advisory board, and Roche advisory board; R. Thiebaut: Employee of IntegraGen SA, Evry, France; F. Liebaert: Employee of IntegraGen SA, Evry, France; S. Falk: reports an Advisory board for Merck pharmaceuticals; J.W. Valle: reports personal fees and non-financial support from Merck J. Hornbuckle: reports non-financial support from Merck UK; T. Iveson: reports personal fees from Advisory Board for Merck Serono; T. Hickish: reports personal fees from Roche, Amgen, Sanofi Aventis, Novartis, Merck Serono advisory boards; D. Cunningham: reports grants from Roche, Amgen, Celgene, Sanofi, Merck Serono, Novartis, Astra Zeneca; T.S. Maughan: reports income from advisory board for Merck;J.N. Primrose: reports personal fees from Merck advisory board, Bayer advisory board, Sanofi-aventis advisory board, and Roche advisory board. All other authors have declared no conflicts of interest.