Effects of insulin,glucagon and neuropeptide Y on expression of SCD mRNA in monolayer primary cultured neonatal calf hepatocytes in vitro

To order to investigate the regulation role of the insulin(In),glucagon(GLN),and neuropeptide(NPY) in hepatocyte glycometabolism and lipometabolism of dairy cows,effects of In,GLN and NPY on expression of stearoyl-CoA desaturase(SCD) mRNA in neonatal calf hepatocytes cultured in vitro were observed by real-time quantitative PCR.The production of SCD mRNA increased along with increase of levels of In,and there is a significant difference amongall group(P0.05);when increased gradually glucagon in the culture media,the production of SCD mRNA decreased,and there is extremely significant difference among all group(P0.01);the SCD mRNA express of hepatocytes is increased by NPY ranged from 0 to 1 000 ng/L(P0.05),and there is significant difference among other group except treated with 50 ng/L and 500 ng/L(P0.05).These results demonstrated that In and NPY in the medium can directly increase the expression of SCD mRNA,but glucagon can depress it,so the three factors can regulate the expression of SCD mRNA in the neonatal calf hepatocytes in vitro.