Membrane-associated nucleobase-functionalized beta-peptides (beta-PNA) affecting membrane support and lipid composition.

Protein-membrane interactions are essential to maintain membrane integrity and control membrane morphology and composition. Cytoskeletal proteins in particular are known to interact to a high degree with lipid bilayers and to line the cytoplasmic side of the plasma membrane with an extensive network structure. In order to gain a better mechanistical understanding of the protein-membrane interplay and possible membrane signaling, we started to develop a model system based on beta-peptide nucleic acids (beta-PNA). These beta-peptides are known to form stable hydrogen bonded aggregates due to their helical secondary structure, which serve to pre-organize the attached nucleobases. After optimization of the beta-PNA solid phase peptide synthesis and validation of helix formation, the ability of the novel beta-PNA to dimerize and interact with lipid bilayers was investigated by both fluorescence and circular dichroism spectroscopy. It was shown that duplex formation occurs rapidly and with high specificity and could also be detected on the surfaces of the lipid bilayers. Hereby, the potential of a beta-PNA-based peptide system to mimic membrane-associated protein networks could be demonstrated.