Protective regulation of the ACE2/ACE gene expression by estrogen in human atrial tissue from elderly men

Data from animal experiments and clinical investigations suggest that components of the renin-angiotensin system are markedly affected by sex hormones. However, whether estrogen affects human atrial myocardium has not been investigated yet. In this study, we determined the effects of estrogen on key components of atrial renin-angiotensin system: angiotensin-converting enzyme, responsible for generation of angiotensin II and angiotensin-converting enzyme 2, counteracting majority of AngII effects, and different renin-angiotensin system receptors, AT1R, AT2R, and MAS. First, the expression levels of estrogen receptors mRNA were determined in right atrial appendages obtained from patients undergoing heart surgery. The amounts of estrogen receptor α and estrogen receptor β mRNA were similar between women (n = 14) and men (n = 10). Atrial tissue slices (350 µm) were prepared from male donors which were exposed to estrogen (1–100 nM; n = 21) or stimulated at 4 Hz for 24 h in the presence or absence of 100 nM es...