STUDIES OF ELECTRICAL IMPEDANCESPECTROSCOPY OF DIABETIC AND NONDIABETIC BLOOD USING 532 NM WAVELENGTHLASERS IN LOW LEVEL LASER THERAPY FOROPTIMAL BLOOD STIMULATION

The study explores the electrical impedance spectroscopy (EIS) measurement procedure to elucidate appropriate irradiation conditions for low-level laser therapy for diabetic blood and biostimulaation effects. The measured EIS error increases with blood dielectric parameters variations and the shift range of polarization. Indeed, the blood dielectric parameters depend not only on the depletion of the cell membrane but also on the polarization in erythrocytes and plasma. Blood dielectric spectroscopy (diabetic of glucose range 7.8-11.3 mmol/L and non-diabetic) were analysed using Impedance analyser (Agilent 4294A) within frequency range 40 Hz-30 MHz. A 532 nm wavelength portable laser (diode-pumped solid-state laser) at 50 mW power output was used for the irradiation. The results showed that the characterized diabetic blood impedance at 50 mW power output within 10 to 15 minutes of irradiation rejuvenates the deteriorated blood and attains optimal biostimulation efficacy also improved erythrocytes deformibility. The electrode polarization (α-dispersion range) extended from frequency 40 Hz to range 11.7-11.9 kHz akin to normal blood. There is higher impedance experienced in the cytoplasm and blood plasma therefore appropriately moderates glucose concentration prevents efflux of K+ and calcium ions. This reduces blood coagulation process and the morphology of the bloat cells to an apt biconcave-like shape.