131 COMBINED EFFECT OF A PHOSPHODIESTERASE 5 INHIBITOR, UDENAFIL, WITH AN ANTIMUSCARINIC, OXYBUTYNIN ON HUMAN DETRUSOR RELAXATION

Human bladder samples were obtained from 15 different patients with no known OAB undergoing cystectomy for bladder cancer. Detrusor strips without urothelium were mounted isometrically at a resting tension of 500 mg in a 5 ml organ bath filled with Krebs-HEPES buffer maintained at 37°C and bubbled w ith 95%O2-5%CO2. The strips were connected to force transducers for isometric tension recordings (Pioden Controls Ltd, UK). Following amplification, the tension changes were computerized via MacLabTM/8 using ChartTM 5 software (AD Instruments Ltd).