The expression and pathogenesis of angiotensin II in EAM mice

The study designed to detect the expression of angiotensin Ⅱ(ATⅡ)in experimental autoimmunemyocarditis(EAM)mice,and explore the role of ATⅡ in the occurrence of EAM.Firstly,EAM model were inducedand constructed.Then,mice were divided into control group,EAM group,and EAM+losartan group.Twenty-onedays after,the mice was sacrificed.The lymphocytes infiltration of model mice was detected by HE staining,them RNA levels of IL-1β,IL-6 and TGF-β were assayed by RT-q PCR,and the ATⅡ expression was assayed byELISA.Transwell was applied to assay the migration ability of macrophages.Data showed ATⅡ expression wasup-regulated in EMA mice,demonstrating the function of promoting the release of inflammatory factors and themigration of macrophages.Losartan(ATⅡ inhibitor)attenuated myocarditis,reduced lymphocyte infiltration,andinhibited the macrophage migration in EAM mice.Furthermore,the expression of IL-1 and IL-6 in losartantreatment group was lower than that of EAM group,but the expression of TGF-β was higher than EAM group.Inconclusion,AT Ⅱ might be contribute EAM development,and AT Ⅱ blockage would attenuate the myocarditisdevelopment.