Preparation of β-cyclodextrin sulfate-immobilized hydrophilic vinyl-polymer gel as a selective, high recovery and stable adsorbent for high-performance affinity chromatography of heparin-binding substances

β-Cyclodextrin sulfate having heparin-mimicking activity was immobilized on a hydrophilic vinyl-polymer gel, TSKgel AF-Epoxy, TOYOPEARL 650M. A column packed with this material could be used for high-performance affinity chromatographic separation of heparin-binding substances such as growth factors, enzymes, coagulation factors and lipoproteins. The substances retained on the column were recovered more easily than those on a heparin-immobilized column. Furthermore, the β-Cyclodextin sulfate-immobilized gel was superior in stability to the heparin-immobilized gel when exposed to acidic and basic solutions.