Biophoton emission of MDCK cell with Hydrogen Peroxide and 60 Hz AC magnetic field

We studied biophoton characteristics of Madin-Darby canine kidney (MDCK) cells under the influence of H 2 O 2 by employing a photomultiplier tube (PMT) and a fluorescence microscope. H 2 O 2 was used for producing reactive oxygen species (ROS) in the measurement. Images from a fluorescence microscope show an increase of photon intensity emitted from the sample due to H 2 O 2 . By using a PMT we measured quantitative change in biophoton emission with application of H 2 O 2 to the MDCK cell culture, found that the increase of the biophoton is dependent upon the amount of H 2 O 2 . The agreement between the results of the PMT and the fluorescence microscope suggests the possibility of quantitative measurement of the influence of ROS on living tissue or cell. In addition we applied a 60 Hz AC magnetic field on the cells to investigate the change in reaction between MDCK cell and ROS. It showed that a decay of chemiluminescence intensity has taken a different path following exposure to the magnetic field. As a result, the PMT measurement might be considered as a useful tool for studying biochemical characteristics in relation to ROS.