Phenotypic cofirmatory disc diffusion test (PCDDT), double disc synergy test (DDST), E-test OS diagnostic tool for detection of extended spectrum beta lactamase (ESΒL) producing Uropathogens

Background The aim of this study was to probe the best diagnostic tool for the detection of Extended Spectrum beta lactamase ES beta L producing uropathogen and their antimicrobial susceptibility profile to treat the infections properly Methods Clinical samples of urine were cultured on Cysteine Lactose Electrolyte Deficient CLED Agar medium Antimicrobial sensitivity tests were carried out by Kirby Bauer disc diffusion method Phenotypic methods were used for further confirmation of beta lactamase production by phenotypic confirmatory disc diffusion test PCDDT double disc synergy test DDST and by E test for ESBL production Aswab on Mueller Hinton MH agar plates was used for further studies and the Optical Density O D of the cultures was set to at nm Results Sample size N was selected from patients suffering from UTI Out of the samples n samples yielded Aerobic Gram Negative Bacteria AGNB The commonest organism isolated was E coli n best antimicrobial result of was shown by imipenem Among the AGNB isolates organisms were ESBL producers E coli showed highest ESBL production of The most effective antimicrobial in ESBL producers was Imipenem Augmentin was least sensitive Conclusion ESBL production is a common phenomenon in UTI patients and screening by DDST for these enzymes is a good epidemiological tool to assess the overall situation in a certain setup It was also seen that Imipenem Carbapenem is the drug of choice nbsp