Matrine alleviates lipopolysaccharide-induced intestinal inflammation and oxidative stress via CCR7 signal

// Guojun Wu 1, * , Wenhong Zhou 2, * , Junfeng Zhao 3 , Xiaohua Pan 1 , Yongjie Sun 1 , Hao Xu 1 , Peng Shi 1 , Chong Geng 1 , Ling Gao 4 , Xingsong Tian 1 1 Department of Breast and Thyroid Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, P. R. China 2 Department of Nursing, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, P. R. China 3 Traffic Police Department, Jinan Public Security Bureau, Jinan 250021, Shandong, P. R. China 4 Scientific Center, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, P. R. China * These authors contributed equally to this work Correspondence to: Ling Gao, email: gaoling8822@sina.com Xingsong Tian, email: xingsong_tianxs@sina.cn Keywords: matrine, inflammation, oxidative stress, CCR7, LPS Received: December 10, 2016      Accepted: December 27, 2016      Published: January 11, 2017 ABSTRACT The aim of this study was to investigate the protective effects of matrine on lipopolysaccharide (LPS)-induced inflammation and oxidative stress in vivo and in vitro . The results showed that matrine improved intestinal inflammatory status and oxidative balance and enhanced chemokine receptor 7 (CCR7) expression. In LPS-challenged mice and Caco-2 cells, matrine alleviated LPS-induced inflammation and oxidative stress via downregulating pro-inflammatory cytokines (IL-1β and IL-17) and malondialdehyde (MDA) production. CCR7-siRNA transfection blocked the protective effects of matrine on LPS-induced inflammation and oxidative stress and exacerbated LPS caused injury. In conclusion, matrine alleviates LPS-induced intestinal inflammation and oxidative stress in mice and Caco-2 cells, which may be associated with CCR7 signal.