Rosa Rugosa Flavonoids Alleviate Myocardial Ischemia Reperfusion Injury in Mice by Suppressing JNK and p38 MAPK

Objective Although Rosa rugosa has been applied for preventing coronary artery disease, the pharmacological mechanism is little explored. In this study, the effects and mechanisms of Rosa rugosa flavonoids (RRF) on myocardial ischemia reperfusion injury (MIRI) were investigated. Methods Mice were pretreated by intragastric administration of 600 mg/kg RRF for 7 days. Then MIRI was induced by 45 min coronary artery ligation and 3 h reperfusion. Myocardial infarct size (MIS) and histopathology, activities of myocardial enzymes and effects of RRF on inflammation and apoptosis were evaluated. Results Pretreating the mice with RRF significantly reduced MIS and inhibited activity of plasma myocardial enzymes. Activity of the enzymes associated with anti-oxidation, SOD and TEAC, and mRNA expression of NOX2 were significantly elevated. RRF pretreatment significantly decreased the translocation of p65 from the cytoplasm into the nucleus and reduced the expression of the pro-inflammatory cytokines, IL-6 and IL-1β. RRF pretreatment also significantly prevented the expression of caspase-3 and Bax, and increased the expression of Bcl-2. And RRF inhibited the phosphorylation of JNK and p38 MAPK. Conclusion RRF significantly inhibited MIRI through anti-oxidative, anti-inflammatory and anti-apoptosis effects, and mechanisms were associated with its inhibition on phosphorylation of JNK and p38 MAPK. This article is protected by copyright. All rights reserved.