Implementing the Agencourt SprintPrep384 Protocol at JGI

Author(s): Wilson, Steven E.; Richardson, Paul; Chen, Feng; Jett, Jamie; Hammon, Nancy; Kubischta, Duane; Lawrence, Diana | Abstract: Implementing the Agencourt SprintPrep384 Protocol at JGIPresenting Author: Steven E. Wilson Contributing Authors: Paul Richardson, Feng Chen, Jamie Jett, Nancy Hammon, Duane Kubischta, Diana Lawrence U.S. DOE Joint Genome Institute 2800 Mitchell Drive, Bldg. 100 Walnut Creek, CA 94598 sewilson@lbl.gov(925) 296-5769 SprintPrep DNA isolation is a process that allows large fragments of DNA and vectors to be isolated from the host E. Coli cell. Agencourt has developed SprintPrep reagents and semi-automated methods for performing the necessary protocol. Last year, JGI implemented a 96 well SprintPrep method. This year, JGI has made the 384 SprintPrep method virtually user-independent. Moving from the 96 well fosmid isolation method to the 384 well format has led to cost savings due to reagent reductions and a doubling in sequencing throughput. The increase in throughput will lead to an increase in sequencing depth and data confidence.