Cloning of BVDV Structural Protein Gene and Predictions of B Lymphocyte Epitopes of Structural Protein

【Objective】On the basis of immunological bioinformatics and reverse vaccinology,the secondary structure of the BVDV structural protein(SP) and its B lymphocyte epitopes with specificity of antigen were predicted and evaluated comprehensively,and some potential antigenic determinants were worked out from the SP gene sequence of the strain with related software and methods of bioinformatics.【Method】The structural protein(SP) gene of the bovine viral diarrhea virus(BVDV) was amplified by RT-PCR and then sequenced.The secondary structure,flexibility,hydrophilicity,accessibility and antigenicity of pl were analyzed by using DNA Star Protean software,and the B cell epitopes were predicted.【Result】The results showed that there were several domains in P14,gp48 and gp53 which belonged to predominant B cell epitopes and a few of B cell epitopes were in gp25.【Conclusion】 Compared with the epitopes that have been published,the results predicted by this method had high reliability and this method was a more advanced epitope research approach.