Abstract B99: Antimetastatic and apoptosis induction potential of supercritical CO2 extract of Nigella sativa seeds

Nigella sativa Linn. is a famous spice that has been used as a medicinal plant for centuries. Traditionally, it is known to have a magic healing power for different varieties of illness, range from intestinal disturbances to fever to cancer. In this study, essential oil was extract from seeds of Nigella sativa using supercritical CO 2 extraction system at extraction pressure of 2500 psi and temperature of 60°C (these extraction conditions of pressure and temperature were reported from our preliminary screening anti-proliferative study against various human cancerous cell lines). The anti-metastatic and anti-invasive properties of the extract were studied using a panel of assays against human breast cancer cell line (MCF 7). Results revealed that the extract showed a potent anti-proliferation activity against MCF 7 cells with IC 50 value of 53.34 ± 2.15 μg/ml once tested using MTT assay. The extract also halted MCF 7 cells migration and invasion, where 40 μg/ml of the extract A3 represented 49.80 ± 5.89% of migration inhibition and 79.29 ± 2.09% of invasion inhibition. In addition, the extract significantly induced apoptosis of the MCF 7 cells by activating caspases 3/7 and 8. The result of this study revealed the role of supercritical CO2 extracted oil of Nigella sativa in prevention of metastasis and invasion of breast cancer. Note: This abstract was not presented at the conference. Citation Format: Hussein M. Baharetha, Zeyad D. Nassar, Abdalrahim F. Aisha, Abd Kadir, Zhari Ismail, Abdul Majid. Antimetastatic and apoptosis induction potential of supercritical CO2 extract of Nigella sativa seeds. [abstract]. In: Proceedings of the AACR Special Conference on Tumor Invasion and Metastasis; Jan 20-23, 2013; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2013;73(3 Suppl):Abstract nr B99.