Comparison of the Effects of Glycerol, Mannitol, and Urea on Ischemic Hippocampal Damage in Gerbils

The effects of glycerol and mannitol, as well as urea, on delayed neuronal death (DND) in the gerbil hippocampus were investigated. 20% solution of glycerol, mannitol and urea were prepared, and 6.5 ml/kg of each agent, or saline, was administered to male Mongolian gerbils intraperitoneally 30 min before ischemia. The animals were subjected to transient forebrain ischemia for 5 min. Seven days after the ischemic insult, the brains were fixed and stained for histopathological analysis. The number of normal neurons (neuronal density, ND) in a 1mm linear length of hippocampal CA1 region was counted. ND of sham-operated group (n = 6) was 275.3 ± 16.7 (mean ± SD). ND in the saline-treated group (n = 6) was 14.8 ± 5.0. ND of groups treated with glycerol (n = 6), mannitol (n = 6) and urea (n = 4) was 68.2 ± 56.7 (p < 0.01), 52.8 ± 54.4 (p < 0.01) and 12.0 ± 2.5 (NS), respectively. The present study demonstrates that glycerol and mannitol have some protective effects against DND in the gerbil hippocampus, whereas urea has no effect.