Study of temperature effect on single-cell fluid-phase endocytosis using micro cell chips and thermoelectric devices

In this work, an array-type micro cell chip with micro temperature sensors (iMTS) and a thermoelectric device was fabricated to investigate temperature effect on fluid-phase endocytosis (FPED) of HeLa cells at the single-cell level. Using two different molecular probes, large-scale single-cell FPED data (more than 500 cells) demonstrated the existence of a critical endocytosis temperature of 16-17oC for HeLa cells. Activation energy at higher temperature range ( 18 kcal/mol). This energy difference suggests that endocytosis can occur through different mechanisms at different temperatures.