Abstract 118: Effect of Interferon Regulatory Factor-1 on Vascular Dementia Using Mouse Chronic Cerebral Hypoperfusion Model

Objective: Accumulation of inflammatory cells around the damaged blood vessels is an important feature of the pathophysiology of Binswanger disease due to hypoxic conditions. Recent reports indicate that expression of interferon regulatory factor (IRF)-1-dependent genes in neurons plays a role in ischemic neuronal death. However, the roles of IRF-1 in vascular dementia are not well investigated. Therefore, we assessed the effect of IRF-1 on cognitive function using vascular dementia mouse model by chronic cerebral hypoperfusion. Methods: Male 10-week-old C57BL/6 (wild-type; WT) and IRF-1-knockout (IRF-1KO) mice were used in this study. Chronic cerebral hypoperfusion mouse model was generated by bilateral common carotid artery stenosis (BCAS). Six weeks after BCAS, the mice were subjected to the Morris water maze test 5 times a day for 5 days. After the Morris water maze task, cerebral blood flow (CBF) was evaluated by laser speckle flowmetry. Expression of NADPH oxidase subunits and inflammatory cytokines...