New method to preserve the original proportion and integrity of urinary cell-free DNA

Background Due to high nuclease activity and complex contents in urine, urinary cell‐free DNA (ucfDNA) was prone to degrade. So, we developed standardized urine collection tube (UCT) to prevent ucfDNA degradation and simultaneously maintain urinary cells in their original form during the sample collection process, ensuring stabilization of the original proportion and integrity of ucfDNA.