The Binding Performance of Erythromycin Imprinted Polymeric Microspheres

Erythromycin molecularly imprinted polymeric microspheres（EM-MIPMs) which have good shape and well-distributed size were prepared by suspension polymerization in aqueous phase, using EM as a template and methacrylic acid (MAA) as a functional monomer. The size of MIPMs is about 50.17 μm characterized by laser particle size analyzer and polarizing microscope. The interaction between the template and monomer, and selective binding performance were investigated by ultraviolet spectrum analysis, equilibrium binding experiments and Scatchard analysis. The results show tha t hydrogen bonding may contribute to the interaction between EM and MAA, EM-MIPMs demonstrated much higher adsorption capacity compared with the NMIPMs. It has also been found that there are two kinds of binding sites in EM-MIPMs and only one kind in NMIPMs.