Synthesis of Proteins by Automated Flow Chemistry
2020
Ribosomes produce most proteins of living cells in seconds.
Here we report highly efficient chemistry matched with an automated fast-flow
instrument for the direct manufacturing of peptide chains up to 164 amino acids
over 328 consecutive reactions. The machine is rapid - the peptide chain
elongation is complete in hours. We demonstrate the utility of this approach by
the chemical synthesis of nine different protein chains that represent enzymes,
structural units, and regulatory factors. After purification and folding, the
synthetic materials display biophysical and enzymatic properties comparable to
the biologically expressed proteins. High-fidelity automated flow chemistry is
an alternative for producing single-domain proteins without the ribosome.
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