Spiribacter aquaticus sp. nov., a novel member of the genus Spiribacter isolated from a saltern
2017
A moderately halophilic bacterium, designated strain SP30T, was isolated from a solar saltern located in Santa Pola, Alicante, on the East coast of Spain. It was a Gram-stain-negative, strictly aerobic bacterium, able to grow in 7.5–25 % (w/v) NaCl and optimally in 12.5 % (w/v) NaCl. Phylogenetic analyses, based on 16S rRNA gene sequences, showed that the novel isolate is a member of the genus
Spiribacter
, with the most closely related species being
Spiribacter roseus
SSL50T (99.9 % sequence similarity) and
Spiribacter curvatus
UAH-SP71T (99.4 % sequence similarity). The 16S rRNA gene sequence similarity with the type species
Spiribacter salinus
M19-40T was 96.6 %. The DNA–DNA relatedness value between strain SP30T and
S. roseus
SSL50T and
S. curvatus
UAH-SP71T was 40 and 55 %, respectively; these values are lower than the 70 % threshold accepted for species delineation. The major fatty acids were C16:0, C18 : 1ω7c, C19 : 0 cyclo ω8c and C12 : 0. Similarly to other species of the genus
Spiribacter
, strain SP30Twas observed as curved rods and spiral cells. Metabolic versatility was reduced to the utilization of a few organic compounds as the sole carbon and energy sources, as with other members of
Spiribacter.
However, it differed in terms of colony pigmentation (brownish-yellow instead of pink) and in having a higher growth rate. Based on these data and on the phenotypic, genotypic and chemotaxonomic characterization, we propose the classification of strain SP30T as a novel species within the genus
Spiribacter
, with the name Spiribacter aquaticus sp. nov. The type strain is SP30T (=CECT 9238T=LMG 30005T).
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