Role of sToll-like receptors 2 and 4 in stage 2 periodontitis patients with and without type 2 diabetes: A Randomized clinical control trial

Objective: The role of TLRs as principal signaling receptors in recognizing endotoxins on gram positive and negative bacteria is facilitated by TLRs and further enhances its role as a potential biomarker in assessing periodontal disease. The study aimed at evaluating the expression of sTLR2 and sTLR4 among healthy, periodontal and diabetic patients and further if there a comparable expression among the TLRs among genders. Material and Methods: Patients were selected according to pocket depth, Clinical attachment loss and radiographic bone loss. Unstimulated whole saliva was collected. sTLR2 and sTLR4 quantification was estimated by ELISA. Comparison of sTLRs between the study groups were performed using ANOVA followed by Tukey post Hoc test. Independent sample t test was used to compare between the genders. (p≤0.05) Result: Expression of sTLR2 was higher among periodontitis patients compared to diabetic patients, this was also evident with relation to genders. sTLR4 showed significant expression among the three groups and also among the genders. Conclusion: The expression of sTLR2 was higher among periodontitis patients compared to diabetic patients. More studies need to be carried out to evaluate TLRs values among genders.