The effects of temperature changes on the isozyme and Hsp70 levels of the Amur sturgeon, Acipenser schrenckii, at two acclimation temperatures

Abstract The isozyme spectrum expressions and Hsp70 expressions in the muscle and the isozyme activities in the liver were measured to evaluate the stress response of the Amur sturgeon, Acipenser schrenckii, to rapid temperature changes at two acclimation temperatures. The fish (body weight 6.18 ± 0.21 g) were subjected to an increasing temperature change (from 13 °C to 23 °C, Tinc treatment, 60 individuals) or to a decreasing temperature change (from 23 °C to 13 °C, Tdec treatment, 60 individuals) for 72 h. After 0 h, 1 h, 3 h, 12 h, 24 h, 48 h and 72 h of exposure, the fish (n = 7) were removed and prepared for further analysis. The results showed that the levels of Hsp70 in the muscle were significantly upregulated after 48 h of heat exposure (P