Screen of Combination of Radioprotective Agents

Objective To screen the best combination of radioprotectors by pretreating and curing cobalt-60 gamma irradiated mice with combined.Methods Synthetic compounds(E0703,523) were used as preventive agents,cytokine(rhG-CSF,rhIL-11) was used to cure early acute radiation damage,and tradiational Chinese medicine formulas(new si-wu capsules,ci-bai capsules) were used to reverse late radiation damage.Ninety mice were irradiated at a dose of 7.5 Gy using cobalt-60 gamma resources.Blood samples were collected from the tail end and the number of peripheral blood cells was counted on day 0,1,4,7,10,13,16 and 22 using a microcell counter.Hematopoietic progenitor colony-forming cell assays were used to assess the reconstitution of bone marrow after radiation-induced myelosuppression.Results Compared with control,white blood cells,red blood cells,hemoglobin and platelets were all increased in drug-treated groups,except for group Ⅶ.GroupⅠand group Ⅲ significantly increased the formation of hematopoietic progenitor colony-forming cell assays.Group Ⅲ also accelerated blood cell recovery.Group Ⅴ led to a significant increase in the formation of colony-forming unit-granulocyte macrophage and circulating blood cells.Conclusion Group Ⅲ is the best combination of radioprotectors.