Sporophytic photosynthesis and gametophytic growth of the kelp Ecklonia stolonifera affected by ocean acidification and warming

Juvenile sporophytes and gametophytes of Ecklonia stolonifera were incubated in combinations of three pCO₂ levels (360, 720 and 980 ppmv) and two temperatures (10 and 15°C for sporophytes; 15 and 20°C for gametophytes) to examine potential effects of climate change on photosynthesis and growth. Sporophytes had significantly higher maximum quantum yields (Fᵥ/Fₘ) and maximum relative electron transport rates (rETRₘₐₓ) at 720 ppmv than 360 and 980 ppmv. Also, these parameters were significantly lower at higher temperature of 15°C than at 10°C. Growth of female gametophytes was maximal at 360 ppmv rather than enriched pCO₂ levels. Female gametophytes had significantly lower growth at higher temperature of 20°C than at 15°C. These results indicate effects of elevated pCO₂ varied between generations: stimulating sporophytic photosynthesis and inhibiting gametophytic growth. Ocean acidification and warming would constitute a grave threat to seedling cultivation of E. stolonifera caused by growth inhibition of gametophytes at high pCO₂ levels and temperatures.