Effect of seeds of Entada phaseoloides on chronic restrain stress in mice

Abstract Background Entada phaseoloides is a well-known medicinal plant traditionally used in Ayurvedic medicine for centuries. Objective To evaluate the anti-stress activity of seeds of E. phaseoloides in endoplasmic reticulum stress during chronic restrain stress in mice, based on our preliminary screening. Materials and Methods Mice (n = 6/group) were restrained daily for 6 h in 50 ml polystyrene tubes for 28 days. Methanolic extract of E. phaseoloides (MEEP) (100 and 200 mg/kg, p.o.) and standard drug, imipramine (10 mg/kg i.p.) were administered daily 45 min prior to restrain from day 22–28. Then, forced swim test (FST) was performed to assess despair behavior. Lipid peroxidation (LPO) and antioxidant enzymes Reduced glutathione (GSH), Superoxide dismutase (SOD) were measured in the hippocampus of mice. 78 kDa Glucose-regulated Protein, 94 kDa Glucose-regulated Protein, C/EBP homologous protein, Caspase-12 expression were quantified by Real Time PCR. Results MEEP significantly reduced the immobility time in FST ( P P P P P 1 ), Entadamide A ( 2 ), Entadamide A-beta- d -glucopyranoside ( 3 ) and 1-O-protocatechuoyl-β- d -glucose. Conclusion MEEP altered endoplasmic reticulum stress in chronic restrain stressed mice; however, as an antidepressant it showed a weaker response.