Effect of TNF-α, IL-2, IL-5 and IL-6 on Rat Tracheal and Bronchial Smooth Muscle Contractions

The article addresses the role of TNF-α, IL-2, IL-5 and IL-6 in the contractile activity of rat tracheal and bronchial smooth muscle contractions. The studies were carried out on isolated preparations using electrical stimulation of postganglionic nerves (frequency 30 stim/s, duration 0.5 ms, amplitude 20 V, stimulation duration 10 s). Two groups of animals were formed: experimental rats were sensitized with ovalbumin by its intraperitoneal (i.p.) administration that was repeated after 14 days. Further, the contractile activity of tracheal and bronchial smooth muscles was assessed after repeated ovalbumin administration and perfusion of interleukin solutions. It was found that TNF-α, IL-2, IL-5 and IL-6 lead to a pronounced increase in contractile responses of tracheal and bronchial smooth muscles both in control and ovalbumin-sensitized animals. Among the studied cytokines, the strongest constrictor effect was exerted by TNF-α and IL-6.