Effects of Cycloheximide on DNA and Protein Synthesis in Rat Liver

SummaryCycloheximide markedly decreases protein synthesis (68%) at a time when RNA synthesis is only slightly inhibited. The DNA content of the liver increases with increasing dosage of the drug; animals treated with cycloheximide and having 1.5 times as many nuclei had twice as much DNA-P in their liver as control animals. The abrupt inhibition of protein synthesis contrasts with a gradual, dose-dependent rise in DNA-P and suggests a separation of these two effects of cycloheximide.