Analysis of test results of urinary hydroxyproline and c- terminal telopeptide of collagen type II concentrations among Kashin-Beck disease patients in Qinghai plateau

Objective To detect urinary bio-markers of hydroxyproline (HYP) and c-terminal telopeptide of collagen type Ⅱ (CTX-Ⅱ) among population from Kashin-Beck disease (KBD) regions in Qinghai Province, and to provide the scientific data for prevention and control of adult KBD. Methods According to the "Diagnosis of Kashin-Beck Disease" (WS/T 207-2010), using case-control study, 120 KBD patients (males 55, females 65) and 89 healthy controls (males 41, females 48) in Qinghai KBD regions were divided into case group and control group. Morning urine samples were collected. HYP and CTX-Ⅱ contents were analyzed by enzyme-linked immunosorbent assay (ELISA), then these results were corrected with creatinine. All the data were analyzed by SPSS 17.0 software. Results There was no significant difference in the age of male and female between case group and control group (t= 1.813, 1.131, P > 0.05). The medians of urinary HYP and CTX-Ⅱcontents among male patients were 74.91 μg/μmol Cr and 630.77 ng/μmol Cr, respectively, which were higher than those of control groups (51.38 μg/μmol Cr, 401.32 ng/μmol Cr, Z= 3.068, 3.246, P 0.05), however, the difference in CTX-Ⅱ content was statistically significant (Z= 2.002, P < 0.05). Conclusion The urinary HYP contents of male patients with KBD change significantly, while the degradation of type Ⅱ collagen in male and female patients increases, and CTX-Ⅱ could reflect the metabolic changes of collagen in KBD. Key words: Kashin-Beck disease; Bio-marker; Hydroxyproline; C-terminal telopeptide of collagen type Ⅱ