O11 Application of autofluorescent dermoscopy for evaluation of skin changes in cosmetology

Introduction: Patient selection and choice of best treatment strategy for degenerative skin lesions as well as evaluation of treatment outcomes might be further refined with use of the techniques of objective evaluation of skin characteristics, which is a mainstay of evidence-based medicine. Materials and Methods: We used fluorescent videodermatoscope “EcoSkin” for registration and evaluation of skin fluorescence. Fluorescence was elicited with violet light (400 nm) from lightemitting diode, and registered with TV RGB detector. Specially designed software “Skin-Matrix” was used for analyses. Patient cohort comprised 130 females aged between 13 and 93 years. 72 patients had Fitzpatrick skin type II and 58 type III. For quantitative evaluation of skin characteristics, we used the integral parameter A calculated as A= f(H,G,R/G,B/G). Digital values of GS%-background fluorescence as well as the ratios of fluorescence intensity within different channels such as R/G and G/B were mathematically derived. Coefficient of heterogeneity (H) was calculated with use of special software Flt.exe. Results: Strong positive correlation (r = 0.80 0.85) was found between parameter A and biological age of the patients. However, correlation coefficient was different in the insolated and noninsolated areas. This is suggestive of the influence of a variety of factors on parameter A and confirms different extent of photomediated skin injury. The contribution of photomediated injury was estimated as a difference between the value of parameter A on the insolated skin areas and non-insolated areas. We failed to find significant correlation of photomediated injury with age. Conclusion: The technique can be used for evaluation of agerelated skin changes in cosmetology, diagnostics of the impact of photomediated injury, and monitoring of treatment efficacy.