Microarray Analysis of Toxicogenomic Effects of Sodium Hypochlorite on Pseudomonas aeriginosa

Sodium hypochlorite has been utilized as an antimicrobial on Sodium hypochlorite has been utilized as an antimicrobial on pathogenic bacteria such as pathogenic bacteria such as Pseudomonas Pseudomonas aeruginosa. Previous studies have not been able to elucidate the Previous studies have not been able to elucidate the mechanisms by which sodium hypochlorite impacts mechanisms by which sodium hypochlorite impacts antimicrobial activity, because of the inability to determine th antimicrobial activity, because of the inability to determine the transcriptional responses to the entire genome. The goal of this transcriptional responses to the entire genome. The goal of this study was to determine global gene expression changes in study was to determine global gene expression changes in P. aeruginosa after 20 minute exposure to 4.4 after 20 minute exposure to 4.4 mM mM sodium hypochlorite by means of hypochlorite by means of Affymetirx Pseudomonas GeneChip arrays. Real arrays. Real-time RT time RT-PCR was used to validate data from the PCR was used to validate data from the GeneChip arrays. To our knowledge, this is the first arrays. To our knowledge, this is the first microarray analysis of analysis of P. aeruginosa genome with sodium genome with sodium hypochlorite. The results suggested that sodium hypochlorite hypochlorite. The results suggested that sodium hypochlorite induces cellular protective processes related to secreted factor induces cellular protective processes related to secreted factors s and cellular defenses and represses primary metabolic and cellular defenses and represses primary metabolic pathways related to oxidative pathways related to oxidative phosphorylation and electron and electron transport. This global transcriptional profile can be used to transport. This global transcriptional profile can be used to further advance the understanding of the mechanisms involved further advance the understanding of the mechanisms involved in toxicity of sodium hypochlorite in in toxicity of sodium hypochlorite in P. aeruginosa.