Clinical efficacy of platelet concentrates prepared by improved buffy-coat method

Objective To explore the clinical efficacy and safety of platelet concentrates prepared by improved buffy-coat method. Methods From October 2012 to February 2014, a total of 89 cases of hospitalized patients in a certain hospital in Zhengzhou City who needed platelet transfusion were collected into this study, they were divided into study group (n=49) and control group (n=40) according to different kinds of platelet preparations they transfused. Patients in study group were transfused with platelet concentrates prepared by improved buffy-coat method, and patients in control group were transfused with apheresis platelets. Platelet counts were detected at pre-transfusion, 1 hour after transfusion and 24 hours after transfusion, then corrected count increment (CCI) was calculated. The improvement of bleeding in patients and transfusion adverse events were observed and recorded before and after transfusion. The platelet counts, CCI, transfusion efficacy and the incidence of adverse reactions in two groups were compared with each other by statistical methods. The study protocol was approved by the Ethical Review Board of Investigation in a certain hospital in Zhengzhou City. Informed consent was obtained from all participants. There were no significant differences of the age, gender ratio, type of diseases and platelet counts before blood transfusion between two groups (P>0.05). Results ①There were no significant differences in platelet content, capacity and the residual of white blood cells and red blood cells between apheresis platelets and platelet concentrates prepared by improved buffy-coat method (P>0.05). ②Platelet counts were significantly increased 1 hour and 24 hours after transfusion compared with the platelet counts before transfusion in two groups, and the differences were statistically different (P 0.05). ③There was no significant difference in rate of adverse reactions after platelet transfusion between two groups (P>0.05). Conclusions Platelet concentrates prepared by improved buffy-coat method are safety and efficacy for patients, whether it is worth of clinical promotion and application, still needs large sample and multicenter randomized controlled trials to confirm. Key words: Platelet concentrates; Improved buffy-coat method; Apheresis platelets; Treatment outcome