Sequence polymorphisms of mitochondrial DNA hypervariable 1 of peripheral blood cells from 47 HIV/AIDS patients of Han nationality

Objective To investigate alterations of hyper variable region 1(HR 1)of mitoehondrial DNA (mtDNA)in white blood cells of Chinese Han nationality HIV/AIDS patients.Methods Blood cells were obtained from 47 cases of therapy-na(i)ve HIV/AIDS patients without opportunity infection and DNA were extracted using blood DNA extracted kit.About 600bp fragments which contain HR 1 were amplified by PCR.Alterations were determined by directed DNA sequencing.Results There were 124 polymorphism sites in mtDNA HR 1(nb16024-16383)in 47 HIV/AIDS patients.The alteration rate was 0 to 20.47%(median 5.33%).36 cases experienced C to T nucleotide change at nt 16 223,and the alteration rate was 70.97%.At nt 16 362,26individuals showed T to C nuchotide change and 3 individnals showed T to G alteration,alteration rate was 55.32%(26/47)and 6.38%(3/47)individually.Conclusion HIV infection may cause more alterations in HR 1 regions. Key words: HIV; Mitochondria; DNA; Mutation