ExRNA Sequencing Results of Different Volumes of Serum

Circulating extracellular RNAs (exRNAs) are very important potential biomarkers of disease. But few have been reported the extracellular RNA profiles in serum. In this study, we tried to use RNA-seq technology to analyze the extracellular RNA by using low volume peripheral blood serum and compared the sequencing results between different volumes of serum (500 μl, 1.5 ml, 2.5 ml, 3.5 ml). According to the results, we found that most of the exRNA in the serum is lncRNA, protein_coding and MT-rRNA. And when the volume of serum is between 0.5 - 3.5 ml, the numbers and reads of exRNA, including the reads of circRNA, have not changed significantly. It indicates that the sequencing result of exRNA in serum has no significance with the initial serum volume.