Evaluation of the Effect of Protamine on Human Prostate Carcinoma PC-3m Using Contrast Enhanced Doppler Ultrasound.

ABSTRACTPurpose: We evaluated the ability of contrast enhanced Doppler ultrasound (CEDU) to demonstrate changes in tumor vascularity during angiogenesis inhibitive therapy in PC-3m human prostate carcinoma.Materials and Methods: Pieces of human prostate carcinoma PC-3m tumor (Institute of Urology, Beijing University, Beijing, People’s Republic of China) were transplanted subcutaneously into 48 male BALB/C nude mice (Center of Animal Quarantine, Beijing, People’s Republic of China). Protamine (Shanghai Biochemistry Pharmaceutical Co., People’s Republic of China) was injected subcutaneously as an angiogenesis inhibitor. The animals were randomly divided into 3 groups according to protamine dose. The color flow signal-pixel rate (SPR) of the images was calculated using the number of pixels showing color Doppler signals as a ratio of the total number of pixels covering the lesion.Results: The SPR of the high and low dose groups were significantly lower than that of the control group (p <0.01). Mean SPR ± SD i...