Screening Her2 and Survivin mimic peptides from Ph.D.-7 phage display peptide library

Objective To screen Her2 and Survivin mimic peptides that were associated with bladder cancer from Ph.D.-7 phage display peptide library.Methods Ph.D.-7 phage display peptide library was biopanned for 3 rounds by using Her2 and Survivin polyclonal antibody and then phage titer was detected.Enzyme linked immunosorbent assay (ELISA) dection and competitive supression test were performed to detect the binding force of random-selected monoclonal phages.DNA extracted from phages with better binding activity were sequenced and got the basic group of core sequence that was appeared more often relatively.Then the polypeptide was synthesized and ELISA detection and competitive supression test were performed again to detect the binding force.Results After 3 rounds of biopanning,the titer of phages were increased gradually.Select 20 monoclonal plaque from titering plates of Her2 and Survivin respectively.After the sequence of DNA,the core sequence of her2 was ACT ACG GCT GAG AAT AAT GAG and the sequence of Survivin was TCT CCT CCT CAT CTT TCG CAG.Then polypeptides were synthesized successfully.The sequence of Her2 polypeptide was Thr-Thr-Ala-Glu-Asn-Asn-Glu (TTAENNE) and the sequence of Survivin polypeptide was Ser-Pro-Pro-His-Leu-Ser-Gln(SPPHLSQ).Conclusion Ph.D.-7 phage display peptide library was used to screen Her2 and Survivin mimic peptides successfully,which would supply a good basement and condition for the advanced reseach about polypeptide vaccinum of bladder cancer. Key words: Ph.D.-7 phage display peptide library; Her2; Survivin