Real-Time Label-Free Impedimetric Protein Detection Using Interdigitated Gold Microelectrodes and Flow Injection Analysis☆

Abstract Real-time detection of protein binding has been achieved using an impedimetric technique. The fabricated chips boasting interdigitated gold microelectrodes were docked into a microfluidic cell with a sample injection system to guarantee good performance, repeatability and flow injection analysis. Electrochemical impedance spectroscopy (EIS) was carried out to characterize the surface and its response to protein binding using label-free direct capture assays [1] . Real-time measurements of the binding process were performed to monitor the association and dissociation phases of the target-analyte pair.